In this paper, graphene oxide (GO) was firstly modified by aminosilane coupling agent, and nano silver was loaded by adsorption and reduction. Then, organic-inorganic hybrid microfiltration membrane was prepared with modified GO and hydrophilic polymer such as polyvinyl alcohol (PVA) through the nonsolvent induce phase separation (NIPS) method. The structure of the prepared Ag@mGO and the hybrid membranes were characterized and identified by FTIR, XRD, TEM, SEM, Contact Angle (CA), Porosity (ε) and MeanPore Size (rm).The antibacterial property, filtration performance and antifouling ability of the hybrid membranes were characterized meanwhile bycorresponding measurements.The results show that the Ag@mGO particle size is below 10 nm and the silver loading is 5.45%. The porosity (ε) is 30% ~ 40% and the average pore size (rm) is 0.54 ~ 0.71 μm, which means good hydrophilicity and excellent filtration performance. At the same time, the rejection rate (R) for BSA was above 80.2%, and the diameter of the inhibition zone for E. coli (K12) was above 1.29 cm, which indicated that the prepared hybrid microfiltration membranes also had good resistance to BSA pollution and antibacterial efficacy.