Abstract:In this work, Extraction process of flavonoids from the drynaria rhizome by using microwave radiation produced by mechanical cavitation broken cell walls of plant cell and with response surface and Box-Benhnken design method was optimized qualitative and quantitative standards between yields of flavonoids and influence factors to confirm interactions between factors and best level combination. The antioxidant activty was estimated by the scavenging activitiy against hydroxyl radical with the oxidation of ferrous sulfate-salicylic acid-hydrogen for rhizoma drynariae flavonoids application and the development of functional products provide theoretical parameters and data index. The results showed that the best extractionof flavonoids was ration of liquid to materials 、microwave power micrwavere and microwave time（1:35 g/mL、400W、9min） and achieved the maximum yield of 10.12% (2.50 mg/ml). The above experimental results show that extraction yield was determined by the response surface and quadratic multinomial function model between influence factors, which provides a theoretical basis for the extraction of quantitative and rhizoma drynariae flavonoids has antioxidant activity in vitro which can be used in food and pharmaceutical industry for a natural preservative oxidation reagents by going deep into research and development.