In this paper, Li4Ti5O12@C was prepared by in-situ composite method using glucose as carbon source. The effect of different carbon content on Li4Ti5O12 was investigated. The structure and surface morphology of the composites were characterized by X-ray diffraction and scanning electron microscopy. The electrochemical performance was measured by constant current charge-discharge and electrochemical impedance spectroscopy. The results show that Li4Ti5O12 coated with 3 % carbon is homogeneous and has the best electrochemical performance. Its first discharge capacity is 185.9 mAh/g, and remains 161.5 mAh/g after 50 cycles at the charge and discharge rate of 0.5 C. Its first discharge capacity is 99.9 mAh/g, when disharged at 2.0 C.