In view of the disadvantages of high cost、hash experimental conditions and low utilization rate of raw materials in existing reports. In the paper, 3,4-dihydroxybenzaldehyde was used as raw material, refer to the literature method, and β-(3,4-dihydroxyphenyl) pyruvate borneol ester was synthesized at first, then under the bread’s yeast fermentation liquid and target product Danshensu borneol ester was synthesized. The influence of amount of glucose and β-cyclodextrin、reaction time 、pH were investigated in the paper. When β-(3,4-dihydroxyphenyl) pyruvate borneol ester was 0.5g , And the best conditions were as follow: amount of glucose was 30g , reaction time was 24h, pH was 7, amount of β-cyclodextrin was 1.5g. enantiomeric excess valve of the target product as high as 84.9%,and main configuration was S-DBZ . The reaction conditions of the process in this paper were mild and the cost was low, which was a very valuable process for the production of Danshensu borneol ester.