With the Zn2 coordination ，the luteolin-Zn(II) coordinate molecularly imprinted polymers(MIPs) were synthesized using luteolin as template, acrylamide(AM) as monomer，N，N?- methylene bis ester amide(MBA) as cross-linker and ethanol as solution. And then C-glycosyflavones from Indicalamus leaf in alcohol-water system were separated by solid phase extraction using MIPs. The results showed that when n(luteolin-Zn)﹕n(AM)﹕n(MBA)=1:5:30，the obtained MIP possessed the highest adsorption capacity of luteolin from methanol phase with a value of 103.4mg?g-1，that is 1.71 times of MIP without coordination，13.75 times of blank polymer. The MIP can revealed selective enrichment capability of C-glycosyflavones from Indicalamus leaf in alcohol-water system, the recovery rates of Orientin, Isoorientin, Vitexin and Isovitexin are 91.95%、80.87%、60.43% and 63.63%, the contents are 55.46、48.78、36.45 and 38.38 times of that in crude extract, the total content of the four kinds of C-glycosyflavones is from 0.70% to 32.68%.