Mn/ATP desulfurizers were prepared by precipitation method and calcination activation with attapulgite (ATP) as carrier. The Mn/ATP desulfurizers were characterized by means of XRD, TG, IR, SEM and H2-TPR. The change of desulfurizers was analyzed by thermodynamic calculation in the process of roasting activation. The desulfurization activity of Mn/ATP under normal temperature was investigated in a fixed bed reactor. The results show that the calcination temperature and calcination method have great influence on desulfurization agent. With the increase of temperature, MnCO3 decomposes into MnO2 in static air; When oxygen content decreases, MnO2 gradually decomposes into lower valence manganese oxides; Changing the roasting method, the sufficient oxygen content reduces the activation temperature of the desulfurizer, and inhibits the decomposition reaction of MnO2. The MnO2/ATP prepared by roasting in dynamic air at 400℃ has the maximum desulfurization activity at room temperature of 20℃, and the breakthrough sulfur content is 172 mg/g.