With glycyrrhizic acid, different monomers (methacrylic acid, hydroxyethyl methacrylate and 4-vinyl pyridine) and cross-linkers (divinylbenzene and ethylene glycol two methacrylate) as raw materials, a series of molecularly imprinted polymers (MIPs) were prepared by precipitation polymerization in the mixed solution of 25 mL acetonitrile and 8 mL N,N-dimethylformamide (DMF) with azodiisobutyronitrile as initiator.The MIPs were characterized and tested by FTIR, SEM and BET.The preparation process of MIPs was optimized, and the results showed that when the cross-linker was divinylbenzene, the monomer was methacrylic acid，the molar ratio of glycyrrhizic acid and methacrylic acid was 1:10 and the porogen was the mixed solution of 25 mL acetonitrile and 8 mL DMF, MIPs showed the best adsorption performance for glycyrrhizic acid.The equilibrium adsorption capacity of glycyrrhizic acid was 398.5 mg/g and the imprinting factor was 3.73 in glycyrrhizic acid solution of 5 g/L.The adsorption kinetics and adsorption isotherm of MIPs were studied.The extract of licorice was refined by molecular imprinted solid phase extraction (MISPE),and the mass fraction of glycyrrhizic acid was increased to 73.10% from 11.27%with 87.1% recovery yield.