Continuous UiO-66 membrane was fabricated on alumina support by pre-coating ZrO2 layer and seeding secondary growth. The structure and morphology of membrane were characterized by XRD and SEM. The membrane integrity was examined by testing the permeance of gas under T=298 K and P=0.08 MPa. The effects of operating pressure and operating temperature on the permeation selectivity of UiO-66 membranes were investigated. As a result, pre-coating ZrO2 layer helped to obtain a continuous uniform UiO-66 membrane of about 5 μm in thickness. Reverse permeation properties were found of i-C4H10 and n-C4H10 on UiO-66 membrane. At 25 ?C and 0.08 MPa, the permeation selectivity of the membrane for i-C4H10/n-C4H10 were 3.60, with the corresponding permeance of 4.39?10-7 mol/(m2?s?Pa) and 1.22?10-7 mol/(m2?s?Pa).