The Intermediate 2-chloro-N-(4-sulfamoylphenyl)acetamide(II) was obtained by the reaction of sulfanilamide and chloroacetyl chloride. Ten 1, 2, 3-triazolyl sulfonamides were prepared using a known one-pot procedure starting from (II), sodium azide and terminal alkynes. The target compounds were characterized by FTIR, 1H NMR, 13C NMR and ESI-MS. The minimal inhibitory concentrations (MIC) of target compounds against S.aureus and E.coli were determined with double broth dilution method. The results showed that all the tested compounds exhibited a certain antibacterial activities. In particular, 2-(4-(3-fluorophenyl)-1H-1, 2, 3-triazol-1- yl)-N-(4- sulfamoylphenyl)acetamide (IIIh) possessed similar activity against E.coli compared with the positive control drug (ciprofloxacin 0.25 μg/mL).