Waterborne hydroxyl polyacrylate(hs-WA) was synthesized by semi-continuous solution polymerization,with methyl methacrylate(MMA),butyl acrylate (BA), acrylicacid（AA）and hydroxypropyl acrylate（HPA）as comonomers, the benzoyl peroxide (BPO) as intiator，ethanol as co-solvent and triethylamine(TEA) as neutralizer.Then, the quaternary ammonium salt antibacterial agent 3-(methoxysilyl)propyldimethyloctadecyl ammonium chloride (TSA) was introducedand reacted with hs-WA to synthesize an antibacterial waterborne hydroxy polyacrylate (hs-WAT).The samples were characterized by FTIR, 1HNMR, contactangle, SEM, TG, andDSC.The antibacterial properties of the samples were tested by colony counting method and inhibition zone method.The test results were as follows: the TSA was demonstrated to have chemical linkage reaction with hs-WA;After the introduction of TSA and hs-WA reaction, the surface morphology of the resin has not changed; The water contact angle increased from 78.5o to 87.5o, which showed the improvement in surface hydrophobicity; The introduction of TSA enhanced the sample thermal stability with the decomposition temperature is between 299 °C and 456 °C ;The Tg of the resin decreased from 13.5 °C to 8.3 °C,causing the polymer chains become more flexible. Moreover,it was found thaths-WAT sample films with 4% TSA mass fraction(based on the solid content of the resin) showed higher than 99.9% effective against Gram-negative bacteria (E. coli) and Gram-positive bacteria (S.aureus)respectively and no inhibition zoneformed,which proved thatTSA did not migrate and hs-WAT conformed to non-permeable contact antibacterial mode.