Abstract: Eight types of macroporous resins were compared via static adsorption-desorption methods with adsorption capacity and desorption capacity as dexes. Result indicated that AB-8 resin performed better adsorption and desorption properties to total flavonoids from Empetrum nigrum L. var. Japonicum. Then, the purification process was study by static and dynamic adsorption-desorption experiments. The optimal conditions were as follow: static adsorption equilibrium time was 1.0 h, desorption solvent was 95% ethanol and desorption equilibrium time was 1.5 h. Adsorption isotherm of five components from Empetrum nigrum L. var. japonicum by AB-8 macroporous adsorption resin according with Freundlich model and Langmuir model at different temperatures(25℃、30℃、35℃). The maximum sample volume was 400 mL, the impurities were washed by 5 BV 20% ethanol, and the components eluted with 95% ethanol, elution volume was 5 BV and elution flow rate was 3 mL/min. Finally, the purity of total flavonoids from Empetrum nigrum L. var. Japonicum increased from 49.16% to 89.59% at the optimal experimental conditions. This experiment provides a reference for further study of Empetrum nigrum L. var. japonicum.