Isoliquiritigenin is an important chalcone component with plane-conjugated structure. The 10 μm silica gel was used as the substrate and the isoliquiritigenin was used as the ligand. The new isoliquiritigenin bonded silica gel stationary phases (ISLSP-Ⅰ, ISLSP-Ⅱ) were synthesized by continuous liquid phase reaction and intermediate method, respectively. The synthesized stationary phases were characterized by FTIR spectrum and SEM. The stationary phase ISLSP-Ⅰ was packed in a 50 mm × 10 mm i.d. column 1. The stationary phase ISLSP-Ⅱ was packed in a 30 mm × 10 mm i.d. column 2, and the chromatographic performences of the stationary phases synthesized by the two methods were systematically evaluated. The results showed that the column efficiency of both and the peak shapes are good, and the retentions depend on mass transfer at higher flowrate for the test sample. In the methanol-water solvent, column 2 exhibited a certain reversed-phase chromatographic separation mechanism. The relative deviation of retention times do not exceed 0.9 % for test components after 6 months’ use, indicating that the stabilities of the synthesized stationary phases were good. The test results showed that the two columns have good separation abilities for the multi-component samples of standard mixtures and the Chinese medicine extracts.