A solid phase extraction material with specificity extracting clobetasol propionate of molecular capture was prepared by surface imprinting technique in combination with based on solid phase extraction column sieve plate coated with graphene oxide. The molecular capture(MC) was prepared by using clobetasol propionate as template molecule, 3-Aminopropyltriethoxysilane as monomer and ethyl silicate as cross-linker, respectively. The morphology and structure of the molecular-catcher was characterized by FTIR, FESEM, EDS and TGA. The molecular capture was studied by the re-binding experiments, such as adsorption kinetics, adsorption isotherm, molecular identification, applied to the separation and enrichment of clobetasol propionate in cosmetic. The results showed that the molecular capture has a fast adsorption equilibrium, highly improved imprinting capacity and significant selectivity; the detection limit was 0.04 mg/L (S/N=3). The durability test showed that the MC could be reused more than 4 times by re-binding experiments.