Aluminium sulphate (Al2(SO4)3·18H2O), silica sol, tetrapropylammonium hydroxide (TPAOH) and ammonium nitrate (NH4NO3) were used as the raw materials to synthesize HZSM-5 zeolite with a SiO2/Al2O3 mol ratio of 100. HZSM-5 zeolites were treated by adding TPAOH solution for secondary crystallization after pre-treating with sodium acetate solution. Pt/HZSM-5 catalysts were prepared for propane dehydrogenation by loading Pt on to HZSM-5 zeolites before and after treatment. The sam-ples were characterized by XRD, SEM, XRF, BET, NH3-TPD and H2 chemisorption. The effects of different TPAOH crystallization concentrations on the crystal structure, surface morphology, pore structure, surface acidity and propane dehydrogenation of HZSM-5 zeolites were investigated. The results showed that HZSM-5 zeolite treated with CH3COONa solution was recrystallized in TPAOH solution, which not only formed microporous-mesoporous hierarchical structure, but also modulated the surface acidity of the zeolite. If HZSM-5 zeolite is first pre-treated with CH3COONa and then crystallized for the second time in the presence of 0.3mol/L TPAOH and at 170°C for 24h, the Pt/HZSM-5 catalyst exhibited the highest initial propane conversion and propene selectivity, which were 34.2% and 99.0%, respectively.