Abstract: The blend solutions (C/Z-0、C/Z-1、C/Z-3、C/Z-5) with different mass ratios of chitosan and zein and then according blend films were prepared by solution casting method. The effects of mss ratios on rheological properties of blend solutions, microstructure and thermal properties of blend films were analyzed by rotational rheometer, SEM and DSC. The results showed that the blend solutions of different ratios all had pseudoplastic properties. With the increase of zein content, the consistency coefficient decreased, and the fluid index increased from 0.849 to 0.882. The activation energy of blend solutions increased gradually. The dynamic frequency scanning rheological analysis showed that the storage modulus and loss modulus were dependent on frequency. With the increase of zein content, both values increased, and the crossing point moved towards the low-frequency, which indicated the intermolecular hydrogen bonds was enhanced. In C/Z-1 blend films, chitosan and zein molecules bound tightly and had good compatibility, which also leaded to the improvement of thermal stability of blend films.