Abstract: In this study, the polysaccharides of Rhodiola rosea polysaccharides extracted from the aqueous two phase system of ethanol/ammonium sulphate as a active reagent be treated as the antimicrobial factor, a novel antibacterial films material would be prepared by taking the technology of natural circulation method with the glycerinum as the plasticizer, the gelatin as the filmogen, the calcium chloride as the cross-linking agent, the starch as the Pasting agent and the glass pane as the film carrier. In order to investigate the optimum additive amount of films material, the index of evaluation that it cover the strength of extension, moisture-vapor transmission, the degradation properties and antibacterial activity of films material was be established. The antimicrobial results show that the polysaccharides amounts merely contribute to antimicrobial activity, When the polysaccharide content of rhodiola rosea was 6 mL, it can obtained maximum antibacterial rate presenting to Staphylococcus aureus and Bacillus subtilis 93.04% and 92.04% respectively. The other tests results suggested that the optimum additive amount of preparing the perfect films material should be the polysaccharides 6 mL, the glycerinum 1.5 g, the gelatin 1.0 g and the calcium chloride 1.0 g. The prepared technology of antibacterial and edible films by using the natural circulation is easy to master ,to low cost, to be good antibacterial effect and to be easy degradation, which will be the enormous potential in the field of materials without the pollution to the environment.