Introduced β-cyclodextrin (β-CD) into a mixed casting solution using polyvinyl alcohol (PVA) and quaternized chitosan (QCS) as a film matrix, and then crosslinked three substances in the aforementioned casting solution through a combined crosslinking agent (glutaraldehyde (GA) and melamine (MA)), a series of host-guest complex (CDX-QCSY-PVAY) OH- conductive membranes based on cyclodextrin molecule with full interpenetrating network structure were prepared. The results showed that with the growth of β-cyclodextrin content, the content of —OH increased, and the amount of OH- adsorption by complexing Ca2+ increasesed, cause to the ion exchange capacity(IEC) and conductivity and tensile strength and so on increased, but water uptake, swelling ratio and elongation at break decreased. When the content of β-cyclodextrin was 20%, the comprehensive performance was the best. The water uptake and swelling ratio were 91.3% and 83.4%, separately, the tensile strength and elongation at break were 69.5% and 48.5%. And the ion exchange capacity was 2.64 mmol/g, conductivity can reached 7.1 × 10-2 S/cm at 70 ℃. The conductivity of the above film samples can reached 83% of the original conductivity after soaking for 240 h in an alkaline environment of 6 mol/L KOH solution at 70 ℃, and still maintained good alkali stability.