Cu(x)/TiO2 (x=1, 3, 4, 5, 6, 7; x is the amount of copper loading based on the mass of TiO2 carrier, mass percent/%, the same below) catalysts were prepared by impregnation method using copper nitrate and TiO2 as raw materials. The catalysts were characterized by XRD, XPS, NO-TPD and H2-TPR. The performance of the catalysts for the selective catalytic reduction of NO with NH3 (NH3-SCR) was evaluated in a fixed-bed micro-reactor. The results showed that copper species existed in the form of Cu2O and CuO on the TiO2 carrier. The denitrification performance of catalysts was affected by the amount of copper loading. The Cu(6)/TiO2 catalyst exhibited excellent redox properties and good adsorption/desorption performance for reactants, which showed a good catalytic activity in denitrification reaction even at low-temperature (<300 ℃). When the conversion of NO reached 85% and 95%, the reaction temperature T85 and T95 were 195 ℃ and 218 ℃, respectively. The temperature range for the NO conversion over 95% was from 218 ℃ to 270 ℃ with a width of 52 ℃.