Gemini surfactants containing two hydroxyl groups (abbreviated as 12(OH)-s-12(OH), s = 3, 4, 6, 8, 10 and 12) were synthesized using 1-Bromododecane, 2-methylaminoethanol, 1,3-Dibromopropane, 1,4-Dibromobutane, 1,6-Dibromohexane, 1,8-Dibromooctane, 1,10-Dibromodecane and 1,12-Dibromododecane. The thermal property and surface tension of Gemini surfactants in aqueous solu-tion were characterized. The results indicated that the foaming ability of the Gemini surfactants was measured. The aggregation property of Gemini surfactants in aqueous solution were simulated using dissi-pative particle dynamics (DPD). The Gemini surfactants have good thermal property and foaming property. The critical micelle concentration (CMC) of Gemini surfactants were decreased from 0.62 mmol/L(s = 3) to 0.21 mmol/L(s = 6) with the increase of spacer (s≤6), and the CMC almost keep the same when the spacer was continue increased (s >6). With the increase of Gemini surfactant concentration, the aggregate morphologies in aqueous solution were changed from spherical micelle → rod micelle → layered micelle, eventually to reverse micelle network structure. The length of spacer almost has no efficient on the micelle morphology transition in aqueous solution.