The nano-particles of Zeolitic Imidazolate Frameworks ZIF-8 were prepared by solvent process, and the effects of reaction solvent, temperature and time on the structure and properties of ZIF-8 were investigated systematically. Then the synthetic ZIF-8 particles were used as the carrier of the anti-cancer drug doxorubicin hydrochloride (DOX), and the dissolution in vitro were studied. The results showed that the nano-particles of high crystallinity could be obtained in the solvent of methanol, n-octanol or deionized water, respectively. But in the case of deionized water, the triethylamine needed to be added to promote the deprotonation of Ligands. The optimized yield was achieved at the temperature of 25℃, and the 80% of the reaction processes were completed in the initial 3 hours determined by the turbidity. The synthetic nano-particles had good hydrothermal stability, and were sensitive to the acid environment. The drug loading efficiencies for DOX were from 16% to 20%, and the speed of the dissolution in vitro was faster obviously in acid environment than it in the neutral environment.