On the basis of single factor experiments, the effects of acid type, acid concentration, ultrasonic time, solid-liquid ratio, ultrasonic power and temperature on the extraction of alkaloid salts from lotus leaves were studied by response surface analysis and optimization of extraction methods. At the same time, the purification effect of D101 macroporous resin on the extract was explored. The results showed that the optimum technological conditions of lotus leaf alkaloid salts were as follows: heating temperature 60 C, ultrasonic power 500 W, concentration of hydrochloric acid 0.3%, ultrasonic time 41 min, liquid-solid ratio 27:1, and the yield of lotus leaf alkaloid was 4.12±0.05 mg/g. 70% ethanol was determined as elution concentration. The purity of purified alkaloid salts reached 40.81%.