A gelatin-based adhesive was prepared by the diethanolamine, methyl acrylate, trimethylolpropane and epichlorohydrin were used as raw materials to synthesize the different generation of epoxy-terminated hyperbranched poly (amine-ester) (EHPAE). And then, with gelatin extracted from leather wastes as main material and the different generation of EHPAE are respectively as cross-linking monomer, an environmental-friendly of adhesives for footwear or bags were success-fully synthesized. The structure and relative molecular mass distribution of hyperbranched polymer and adhesive were characterized by FT-IR, 1H-NMR and GPC, moreover, the bonding performance of adhesive were measured. The results showed that the modification effect of EHPAE-III was better than the others, when the amount of EHPAE was 30% of the gelatin, the solid content of the modified adhesive was increased from 11.42% to 30.33%. meanwhile, the shear strength and T-peel strength of the adhesive was improved to 2.216 MPa and 3.375 MPa, respectively, and the water contact angle was 100.9o. The adhesive properties and hydrophobicity of the prepared adhesive were better than the commercial available adhesive, in which the shear strength and T-peel strength are 1.88 MPa and 2.864 N/mm, the water contact angle was 84.8o.