In order to improve the transdermal delivery of CoQ10 (CoQ10), CoQ10-Nanosolve with different concentrations of solid lipids were prepared using caprylic caprate triglyceride (GTCC) as liquid lipid and cetyl palmitate (CP) as solid lipid. Its stability and transdermal properties were studied. The results showed that the addition of solid lipids had no effect on the particle size and storage stability of CoQ10-Nanosolve. The particle size of CoQ10-Nanosolve with different concentrations of solid lipids were about 52nm, and there was no significant change in particle size and appearance after 180 days. FT-IR results showed that there was no chemical reaction between CoQ10 and Nanosolve and it could be encapsulated in Nanosolve well. From the occlusive effects, in vitro transdermal experiments, and laser confocal microscopy, it was found that when the mass concentrations of CP increased to 70% and 95%, CoQ10-Nanosolve could significantly reduce the evaporation of water from the skin and better promote the transdermal absorption of CoQ10，and the fluorescence intensity of the epidermal layer and the dermal layer was significantly enhanced.