ZnO nanoparticles (Nano ZnO) were synthesized via solution combustion method with flavone from Ampelopsis grossedentata as raw material and characterized by FTIR, XRD, SEM and EDS. The antioxidant and bacteriostatic properties of the greenly synthesized Nano ZnO were evaluated by Brand-Williams method,inhibition zone method and minimum inhibitory concentration method. The results indicated that the greenly synthesized Nano ZnO had a hexagonal wurtzite structure with good crystallinity and its average grain size was about 32.7 nm. Moreover, the Nano ZnO had good antioxidant activity. When the mass concentration of Nano ZnO was 2.5 g/L, the scavenging rate of 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH·) reached 98.24 %. Furthermore, the antibacterial activity of greenly synthesized Nano ZnO against Staphylococcus aureus was higher than that of traditional chemically synthesized Nano ZnO. The minimum bacteriostatic concentration of greenly synthesized Nano ZnO was 1.00 g/L, while that of chemically synthesized Nano ZnO was 1.50 g/L.