In this study, a novel glyceryl ether fluorocarbon surfactant（sodium 2, 3-bis-octafluoropentanylglycerol-1-sulfate, BOFPGS） was synthesized through a two-step reaction by using 1H, 1H, 5H-octafluoropentanol and 2,3-dibromopropan-1-ol. The structures of intermediate and product were characterized by Fourier-Transform Infrared spectroscopy（FTIR）, 1H NMR spectroscopy and mass spectrometry（MS）. The surface activity was measured. The results showed that it had good surface tension（22.70 mN/m） and critical micelle concentration（4.16 x 10-3mol/）. In addition, emulsifying experiments demonstrated that BOFPGS possessed excellent emulsifying properties at a low concentration of 0.8 × 10-3 mol/L. Moreover, the cytotoxicity experiment verified that BOFPGS had no obvious cytotoxicity at the concentration range of 8.04 × 10-6~ 1.60 × 10-4 mol/L.