Abstract: A series of Gemini nonionic surfactants were synthesized from 1,12-dodecanol, oleic acid and polyethylene glycol (300, 400, 600, 800, 1000). The structure of the product was confirmed by IR and NMR. In order to evaluate the effectiveness of Gemini nonionic surfactants as solubilizing excipients for injection, the surface tension, solubilization and hemolytic activity of Gemini nonionic surfactants were compared with Tween 80. The results show that the safety and effective index of PODD-1000 is 13 times that of Tween-80, which is more suitable for solubilizing excipients of injection.