A series of 2-disubstituted benzothiazole were synthesized via cyclization coupling of o-aminothiophenol and aromatic aldehyde in the deep eutectic solvents (DESs), which was made up of choline chloride and acetamide. The best yield of 98% were achieved through 0.3 mmol of o-aminothiophenol and 0.36 mmol of aromatic aldehyde at 70 ℃ for 1 h in deep eutectic solvent, when the molar ratio of choline chloride and acetamide was 1:2. The DESs method does not need additional catalyst, which has the advantages of mild reaction conditions, simple operations and wide ranges of substrates. In addition, the yield of product can still keep up to 90% after the DESs were reused for 4 cycles in the model reaction.