Li4Ti5O12/C anode material for lithium-ion batteries were prepared by two-step calcination and sol-gel method using tetrabutyl titanate, lithium acetate, citric acid and bamboo charcoal as starting materials. The microstructure and morphology of the materials were characterized by XRD and SEM. The electrochemical properties of the materials were studied by constant current charge discharge, AC impedance and cyclic voltammetry . The electrochemical properties of the materials were investigated by constant current charge-discharge test, AC impedance and cyclic voltammetry. The results show that the Li4Ti5O12/C has good crystallinity, smooth particle surface and uniform dispersion, and the particle size is 200~300 nm. The first discharge specific capacity of Li4Ti5O12/C is 180.4 mA•h/g at 10 C, and 167.5 mA•h/g after 300 cycles. The capacity retention is 92.8%, which is much higher than 46.9% of Li4Ti5O12. At a high rate of 20 C, the capacity retention of Li4Ti5O12/C and Li4Ti5O12 were 68.9% and 41.3%, respectively.