A zwitterionic Gemini surfactant (GS-S) with sulfate group on the spacer group was prepared from dodecyl dimethyl tertiary amine, 1,3-dichloro-2-propanol and chlorosulfonic acid. The structure of the product was confirmed by infrared spectroscopy and nuclear magnetic resonance hydrogen spectroscopy. And the surface tension, wettability, oil-water interfacial tension, salt tolerance, foamability and emulsification were tested. The results showed that compared with cationic Gemini surfactant (GS-O) with similar structure, GS-S had higher surface activity, good emulsifying ability and foaming performance. The critical micelle concentration (CMC) value of GS-S was only 2.3×10-4 mol/L, and the critical surface tension (γCMC) value was 28.43 mN/m. GS-S could reduce the oil-water interfacial tension to low interfacial tension (1.11×10-2 mN/m). Moreover, GS-S could change the wetting state of water on the paraffin film, the contact angle was reduced from 110° (contact angle of deionized water on paraffin film) to 65°. It exhibited excellent salt resistance (＜35 g/L).