Bacterial cellulose (BC) was produced by fermentation of Komagataeibacter xylinus. The BC membrane was characterized by infrared spectroscopy, X-ray diffraction analysis and scanning electron microscopy. The performance of BC film as substrate for mask was evaluated comprehensively. The results show that the BC membrane has a hree-dimensional network structure with an average diameter of less than 100 nm and a crystallinity of 83.46%, which belongs to typical type I cellulose. Compared with non-woven fabrics and silk mask, BC membrane has superior water holding performance, water vapor transmission performance (2263.54 g/m2?24h) and mechanical properties. The absorption of Vc entrapped in Pickering emulsion (42.36 ?g/cm2) by BC membrane was about 2.21 times that of non-woven mask (19.13 ?g/cm2), 1.54 times that of Silk Mask (27.36 ?g/cm2) within 24 h. And 10.96 ?g/cm2 of nutrients could be transported to the skin within 90 min, which is about 1.8 times that of non-woven fabric (6.08 ?g/cm2), and twice that of silk (5.35 ?g/cm2).