An polyacrylate amphiphilic macromolecule (MBDA copolymer) was prepared using methyl methacrylate (MMA), butyl Acrylate (BA), acrylic acid (AA) and diacetone acrylamide (DAAM). The amphiphilic macromolecule was able to co-assemble with fluorescein in water to form water-based fluorescent emulsion (MBDA/FM co-assembled emulsion). The fluorescent shape memory material (MBDA/FM-ADH film) was obtained by mixing with adipic acid dihydrazide (ADH) and curing at room temperature, which can get a wide range of potential applications in the field of anti-counterfeiting, decoration and fluorescent probes. The method for preparing functional shape memory material by self-crosslinking emulsion at room temperature has the features of simple process, friend-environment, good controllability and suitable for large-scale production. The results showed that the particle size of the co-assembled emulsions remained at 100~200nm and the fluorescence intensity of emulsions increased with the amount of fluorescein within a certain range. The films which have dual functions of fluorescence and shape memory emitted uniform green fluorescence under ultraviolet light (em=514nm) and can recovery almost 100% under T≥ 70 ℃ （Tg=31.9 ℃).