Using isophorone diisocyanate (IPDI) and polytetrahydrofuran ether glycol (PTMG/2000) as the main raw materials, dibutyltin dilaurate (DBTDL), 1,4-butanediol (BDO) and 2,2-di hydroxymethyl-propionic acid (DMPA) as catalyst, small molecule chain extender and hydrophilic monomer to prepare a waterborne polyurethane prepolymer (PPU). Hyperbranched poly(amine-ester) (HPAE) synthesized from methyl acrylate (MA), diethanolamine (DEA) and trimethylolpropane (TMP) as a crosslinking agent. Branched waterborne polyurethane adhesives with different HPAE content are prepared. FT-IR spectroscopy and 1H-NMR spectroscopy were used to characterize the structure of HPAE and HPAE-WPU; TGA and multifunctional material testing machine were used to investigate the thermodynamic and mechanical properties of the film. The results show that when the amount of HPAE is 1%, the comprehensive performance of HPAE-WPU emulsion and film is better. The solid content of the emulsion is 33.67%, the water absorption rate of the film is 11.22%, and the water contact angle is 90.32°; the mechanical properties of the film are better, its tensile strength is 10.33 MPa, and its elongation at break is 533.73%; the modified polyurethane has good bonding properties, and its peel strength is 4.063 N/mm.