PS-g-SMCC micro-plates which are easy to immobilize proteins were prepared by initiating activated polystyrene (PS) micro-plates with acrylamide plasma and grafting succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate(SMCC) connector. The surface composition, structure, morphology and protein adsorption properties were characterized by FTIR, EDS, SEM, contact Angle tester and enzyme calibration. The results showed that acrylamide and SMCC were grafted successfully on the surface of PS micro-plates. When the discharge powers was 500 W and the processing time was 300 s, the grafting effect of acrylamide was the best, and the surface of the micro-plates showed excellent super-hydrophilic ability. When the SMCC concentration was 5 g/L, the maximum protein adsorption capacity of bovine serum protein was 903.08 ng/cm2. The protein adsorption capacity was 2.93 times higher than it of the untreated PS micro-plates, which effectively improved the sensitivity of PS micro-plates in ELISA detection.