Cyanidin-3-O-glucoside (C3G) from black soybean hull was enzymatically acylated with lauric acid to achieve an anthocyanin derivative with improved lipophilic property. Various reaction solvent systems were tested and compared for the efficient acylation of anthocyanin by using crude black soybean hull extract (C3G content 35.8%), purified C3G sample (purity>80%), and C3G standard (purity>98%), respectively, as substrate. With the method developed, the acylation rate of the anthocyanin from crude black soybean hull extract reached 66.59%, which reached 75% of that from purified C3G; the acylated product, cyanidin-3-O-glucoside-lauric acid (C3G-La), reached a purity of 82.39% after simple liquid-liquid extraction, and further to a purity of 97.58% after separation and purification by normal phase silica gel. The stability test showed that C3G-La is more stable than C3G in either neutral or acidic pH environment, while both of them are labile under alkaline and light conditions.