Polyethylene glycol monomethyl ether tartrate (TMPEG) was synthesized from tartaric acid and polyethylene glycol monomethyl ether (MPEG) without preprotection of hydroxyl group in tartaric acid by esterification reaction. A series of nonionic waterborne polyurethanes (TWPU) were prepared by the prepolymer process using the hydrophilic monomer as hydrophilic component. The successful synthesis and introduction of TMPEG were characterized by 1H-NMR, FTIR, TGA, tensile tests. The effects of quality score of hydrophilic monomer TMPEG on the storage stability, mechanical properties, water absorption of TWPU was studied. The results showed that when the mass fraction of TMPEG was 24% (based on the total mass of the prepolymer synthesized by the reaction of TMPEG, polyadipate -1,4-butanediol ester diol, isophorone diisocyanate, trimethylpropane, thylene diamine, the same below), the average particle size of TWPU emulsion was 85 nm, the contact angle, tensile strength, elongation at break of the TWPU film were 84°,7.9MPa, 768%, respectively. The comprehensive performance of TWPU film reached the best.