A hydrogen peroxide fluorescent probe TC-BOR was designed and synthesized based on hemicyanine fluorophore with borate as the recognition group，and its structure was characterized by 1HNMR, 13CNMR and HRMS. The result shows that the product after reaction with H2O2 (TC-OH) fluoresces at 723 nm, with 55-fold NIR fluorescence enhancement, a rapid response within 7 min and low detection limit of 2.27 μmol·L-1. Subsequently, TC-BOR was used for the qualitative and quantitative determination of hydrogen peroxide in food and living cells, which provided a new method for the rapid detection of H2O2 in food.