Four kinds of reactive disperse dyes Ⅰ~Ⅳ were prepared by the diazo coupling reaction in which succinimide ester was introduced into precursors. The data of absorption spectrum, photo-thermal stability, process of supercritical carbon dioxide (scCO2) dyeing wool fibers were tested. The results show that four dyes have good photo-thermal stability, and the maximum absorption wavelengths for dyes Ⅰ, Ⅲ and Ⅳ are observed at the range of 360-370 nm, and the maximum absorption wavelength for dye II has a red shift to 400-450 nm. Thus, the dyes are suitable for scCO2 dyeing. The optimal dyeing conditions for dyes are as follows: 2 h, 80 ℃ and 15 MPa for Ⅰ and Ⅲ, 1.5 h, 80℃ and 15 MPa for dye Ⅱ, and 1.5 h, 80 ℃ and 24 MPa for dye Ⅳ. The soaping fastness to soaping and light of the dyed wool fibers can reach 4-5 grade, and the fixation rate can reach up to 86%. Compared with un-activated precursor, the color fastness to soaping improves 2~3 grades, and the fixation rate increases 63%. Scanning electron microscopy (SEM) and microscopy images show that four dyes have good dyes’ permeability, evenly adhere to the surface and interior of the fibers. The test of mechanical parameters shows that the dyed wool fibers can basically maintain the original mechanical properties.