By introducing the coumarin with excellent photophysical properties into the backbone of ligand H3L as a fluorescent signal unit, a new metal-organic octahedral cage Zn?L was prepared by coordinating with transition metal ion Zn(Ⅱ) for realizing selective recognition of glucosamine via the host-guest hydrogen bonding interaction. The characterization results of high-resolution mass spectroscopy and ultraviolet-visible spectroscopy show that this metal-organic cage compound has a M6L4 octahedral structure. Twelve coumarin groups are uniformly distributed at the vertical positions of the octahedral cage, which has high stability in solution and possesses a large cavity with the opening windows that allows free ingress and egress of the guest molecules with suitable size. 1H NMR titration shows that glucosamine combine with cage structure through multiple hydrogen bonding. High resolution mass spectrometry test suggests that a stoichiometric ratio of 1:2 host-guest inclusion complex is formed between the metal-organic octahedral cage Zn?L and the glucosamine and fluorescence titration spectra fitting shows an association constant 1.6 ? 108 M–2, confirming the strong complexation between host and guest, which is conducive to the selective recognition of glucosamine by the cage structure. Glucosamine molecule quenches the fluorescence intensity of Zn?L by up to 93%, while other 9 common biological carbohydrate molecules can only quench 5-20%, showing a highly selective fluorescence response of Zn?L to glucosamine molecule