In this study, a fluorescent chemosensor based on fluoroboron dipyrrole (BODIPY) (Ⅰ) has been synthesized using 8-hydroxyquinoline-5-carbaldehyde and dimethyl pyridine-2, 6-dicarboxylate as raw materials, and the structure was characterized by FT-IR, 1H NMR, 13C NMR and ESI-MS. The sensing performance of sensor Ⅰ was studied by UV-vis/fluorescence spectrophotometry. The results showed that: sensor Ⅰ showed an obviously “on-off” fluorescence response toward Cu2+ in V(DMF)∶V(water)=1∶4 solution, and the quenching efficiency reached a maximum of 97.7 %, and the detection limits of sensor Ⅰ toward Cu2+ was 5.7×10-8 mol/L. Moreover, complex Ⅰ-Cu2+ showed an obviously “off-on” fluorescence response toward PPi, and the detection limits was 1.9×10-8 mol/L. It shows that the sensor Ⅰ has high sensitivity and anti-interference ability in the recognition and detection of Cu2+ and PPi. Through Job's plot and 1HNMR titration data analysis, it is concluded that the binding stoichiometry of sensor Ⅰ toward Cu2+ and complex Ⅰ-Cu2+ toward PPi were 1∶1. The effective determination pH range sensor Ⅰ toward Cu2+ and PPi was from 5 to 9, and the cycle response test could reach more than 4 times.