Indole-3-acetic acid (IAA) production by microbial fermentation has some advantages such as mild reaction conditions, low energy consumption, no employment of toxic and harmful substances, and low environmental pollution. A bacterial strain FX-02 with higher producing IAA ability was isolated and screened from the vegetable rhizosphere soils, and was identified as Enterobacter hormaechei by morphology, physiological and biochemical characteristics, and molecular biology. The fermentation characteristics of FX-02 for producing IAA were studied in this work. The results showed that the IAA biosynthetic pathway of FX-02 belonged to tryptophan-dependent type. The optimal concentration of tryptophan for FX-02 to synthesize IAA was 10.0 g/L, which also indicated that the tolerant concentration of FX-02 to tryptophan was high, in other words, it had very strong transformation ability of tryptophan to IAA. The best carbon and nitrogen sources and mineral salt for FX-02 to produce IAA were glycerol, peptone and calcium chloride, respectively. When the cultural medium (glycerol 10.0 g/L, peptone 10.0 g/L, tryptophan 10.0 g/L, MgSO4 1.5 g/L and K2HPO4 1.5 g/L) was used, and FX-02 was incubated at 30°C in a shaker at 180 r/min for 72 h, the IAA yield of FX-02 was higher.