In order to improve the stretchability of conductive fibers, a wet spinning method is used to prepare polyurethane fibers (TPU fibers). The fibrous nano-silver-polyurethane conductive fiber (AgNPs-TPU conductive fiber) is prepared by immersing in a silver trifluoroacetate solution and reducing it.Explore the effects of different preparation conditions on the conductivity and stretchability of AgNPs-TPU conductive fibers, and study the tensile sensing properties of AgNPs-TPU conductive fibers. The results showed that the quality score of the selected TPU concentration is 25%, the concentration of the immersed silver trifluoroacetate solution was 20mg/mL, and the immersion reduction is 4 times. The resistance of AgNPs-TPU conductive fiber is 10.79Ω.The tensile strength is 5.14MPa.The elongation at break is 576.18%, and it has good electrical conductivity and mechanical properties. In the case of different tensile strains, AgNPs-TPU conductive fiber can characterize different tensile strain information through the change of the resistance rate value. AgNPs-TPU conductive fiber can realize the stable conversion of the tensile strain signal into an electrical signal and has good tensile sensing performance.