Huperzine A (Hup A)is as an acetylcholinesterase inhibitor used to treat Alzheimer's disease. We present a new the molecularly imprinted polymers( MIP), which can well control the release of Hup A. The MIP were prepared by precipitation polymerization using Hup A as template, methacrylic acid（MAA）as functional monomer, ethylene glycol dimethyl acrylate as the crosslinking agent. The synthesized MIP were characterized by SEM、TGA and FT-IR spectrometer. The performance of MIP was evaluated by static adsorption, dynamic adsorption, in vitro drug release and MTT. The results showed that MIP had specific and nonspecific binding sites to Hup A. Hup A tablets were rapidly released within 1 hour, and the cumulative release rate was more than 90%. MIP release gently after 1h and reach equilibrium near 24h, which can achieve the effect of slow release. The release kinetics conforms to the Peppas model and the release index is n = 0.48, which implies a non Fickian diffusion mechanism. The biocompatibility of MIP was good. The cytotoxicity data of L929 mouse fibroblasts to MIP showed that the cell survival rate was higher than 93%. were prepared by precipitation polymerization using Hup A as template, methacrylic acid（MAA）as functional monomer, ethylene glycol dimethyl acrylate as the crosslinking agent. The synthesized MIP were characterized by SEM、TGA and FT-IR spectrometer. The performance of MIP was evaluated by static adsorption, dynamic adsorption, in vitro drug release and MTT. The resμlts showed that MIP had specific and nonspecific binding sites to Hup A. Hup A tablets were rapidly released within 1 hour, and the cumulative release rate was more than 90%. MIP release gently after 1h and reach equilibrium near 24h, which can achieve the effect of slow release. The release kinetics conforms to the Peppas model, and the release index n = 0.48, which is a non Fickian diffusion mechanism. The biocompatibility of MIP was good. The cytotoxicity data of L929 mouse fibroblasts to MIP showed that the cell survival rate was higher than 93%.