Selenizing DMY was prepared with DMY as substrate and Na2SeO3 as selenizing agent. UV, FTIR, NMR, XRD, TG and atomic fluorescence spectrometer were used to characterize its structure and performance. CCK-8 method was used to detect the effect on HSC-3 cell proliferation, and the scratch experiment was used to study the effect on HSC-3 cell migration. The results showed that the basic nucleus of flavonoids still existed in selenizing DMY and the C-Se bond was newly formed, and the selenium content was 6.54%±0.22%. Both DMY and selenizing DMY have a good inhibitory effect on the proliferation and migration of HSC-3 cells, and the inhibitory effect is positively correlated with the concentration. The half inhibitory concentration (IC50) of DMY and selenizing DMY on HSC-3 cells were 25.27 μg/mL and 21.27 μg/mL, respectively. The selenization of DMY effectively improves the ability of inhibiting the proliferation and migration of HSC-3 cells.