A DES (deep eutectic solvent)-based two-phase system (TP-DES) was designed for simultaneous extraction and separation of flavonoids, terpene trilactones (TTLS), procyanidine (PAC), and ginkgolic acids (GAs) from Ginkgo biloba exocarp in our previous work. EOPO and microporous resin were used to recover hydrophilic and hydrophobic ingredients from TP-DES, respectively. Terpene lactones, flavonoids glycosides, and procyanidins were determined by spectrophotometry, and GAs was determined by HPLC. The contents of active components in each step of recovery process were determined for the calculation of the recovery effect respectively. EOPO solution (the molecular weight was 2650) of 90% (w/w) was used to recover flavonoids, TTLS, PAC, and the recovery rates were 89.44%, 53.27%, and 29.77%, respectively. HYA502B resin was used to recover GAs, and the recovery rate was 93.33%. operation, and have good application prospects.