A series of glucosamide quaternary ammonium salt surfactants (CnDGMAPB, where n represents the hydrocarbon chain length of 8, 10, 12, 14 and 16) were successfully synthesized from glucosactone, N, N-dibutyl-1, 3-diaminopropane, and bromoalkane using two-step method of amine ester reaction and quaternary ammonium reaction. The intermediates and products were characterized by FTIR, 1H-NMR and 13C-NMR. The surface activity, wettability, emulsifying, foam and toxicity of the product were studied. The results showed that CnDGMAPB has good surface activity. The critical micelle concentration (cmc) and molecular cross-sectional area (Amin) decreases, the surface activity efficiency (pC20) and saturated adsorption capacity (Гmax) gradually inceased with increasing the carbon chain length. The surface tension (γcmc) value was between 28-32 mN/m. The contact angle between C12DGMAPB/ C16DGMAPB solution and polytetrafluoroethylene membrane (PTFE) was reduced to 47o within the concentration (0.01-5 mmol/L) range. This kind of glycosylamide quaternary ammonium salt had good wetting and emulsifying properties. The foam volume was very low at 5 min, which belongs to the typical low foam product. Both cell toxicity and acute oral toxicity experiment showed that the CnDGMAPB surfactants were low-toxic products. The antibacterial rate of escherichia coli and staphylococcus aureus can reach 100% at the C12DGMAPB concentration of 0.2 g/L, no irritation to skin, and has good antistatic performance.