In order to solve the problems such as strong pungency and low bioavailability of 6-paradol, the 6-paradol-loaded nanoemulsion was prepared by high pressure homogenization technology. Tween 80 was used as the emulsifier, The effects mass ratio of colza oil content on the particle size、Zeta potential、free fatty acid release rate and bioaccessibility were investigated. The result showed that the particle size of nanoemulsion increased obviously with the increase of rapeseed oil content (p<0.05), but ζ-potential had no significant difference. When mass ratio of colza oil content was too small, that is, the emulsion was too sticky, the digestion rate of oil droplets was slowed down, but the extent of which was not be affected. With the increase of mass ratio of colza oil content, the improvement effect of bioaccessibility was better. The bioaccessibility of 6-paradol increased by 74.70% compared to the control group with mass ratio of colza oil content of 20%.