Water is the source of life, but trace water in organic solvents is considered as contaminate. In recent years, explosions caused by trace water in organic solvents have occurred frequently in factories and laboratories, which have attracted great attention from the society. Thus, a convenient method for assessing the water content in organic solvents is desired, urgently. As an emerging analytical tool, organic fluorescent probes offer high sensitivity, good selectivity, low toxicity, real-time visual monitoring and are commonly used in chemical engineering, food, environment, and other fields. This review summarizes fluorescent probes for detecting water content in organic solvents and their applications in foodstuff and other industries since 2016. It focuses on seven strategies for responding to water, which has certain guidance for the development of water content probes.