Vesicles composed of phospholipids and block copolymers combine the advantages of liposomes and polymers while overcoming their respective disadvantages. In this study, phospholipid - block copolymer hybrid vesicle was assembled from polycholesterol methacrylate - block - dimethylaminoethyl methacrylate copolymer (PCMA-B-PDMAEMA) and phospholipids. The morphology, size distribution and zeta-potential of the phospholipid - block copolymer hybrid vesicle were measured. The cytotoxicity and internalization of selected phospholipid - block copolymer hybrid vesicles were measured using RAW 264.7 mouse macrophages. The cell viability of A3 and A8 co-cultured with macrophages were 93.4±1.1% and 92.1±0.8%, which were relatively higher than other hybrid vesicle samples. These results demonstrate that P2 polymer is more suitable than P1 for preparing hybrid vesicles for drug carrier research. Through fluorescence intensity test, A18 was more easily absorbed by macrophages than A16, and DOX loaded with anti-cancer drug was used to treat human kidney cancer cells (OS-RC-2). When DOX final concentration was 3.00 μmol/L, the inhibition rate of OS-RC-2 cancer cells was 75±1.1%.