Three surfactants of tertiary amine N-oxide (TAO), tertiary amine N-benzyl chloride (TABC), and tertiary amine N-methyl iodide (TAMI) were derived from a new tertiary amine (TA) who was synthesized from α-terpinene by Diels-Alder reaction. Their structures were identified by FTIR and 1HHMR, and their surface properties and bacteriostatic ability were investigated. Tests found that the critical micelle concentration (CMC) of TAO, TABC and TAMI are 3.39×10-3, 2.46×10-3 and 2.37×10-3 mol/L, and their γCMC are 33, 25.2 and 26.4 mN/m, respectively, which was better than the commercial analogue that is dodecyl dimethyl benzyl ammonium chloride (DDBAC). The emulsifying time of TAO (528 s), TABC (341.7 s) and TAMI (271 s) are enough long for them to have a good emulsification ability. Except for TAO, both TABC and TAMI have a good foaming performance and foam stability with the foaming height of 76.62 mm and 87.77 mm, and the foaming stability of 0.85 and 0.87, respectively. In addition, they perform well in calcium hard water stability test. Furthermore, TABC shows the best bacteriostatic effect on Staphylococcus aureus, with 21 mm in the diameter of bacteriostatic zone and 99.99% in the bacteriostatic rates.