In this paper, anionic copolymerization of butadiene and styrene with n-butyllithium (n-BuLi) as initiator and 2, 2-bis (5-methyl-2-tetrahydrofuranyl) propane (BMTFP), ethyl tetrahydrofurfuryl ether (ETE) and ditetrahydrofuranyl propane (DTHFP) as polar regulators was carried out under different dosage of regulator and polymerization temperatures. The microstructure, molecular weight and its distribution of styrene-butadiene copolymer were characterized by 1H-NMR and GPC. The results showed that there was no side reaction in BMTFP polymerization system and the molecular weight distribution of copolymer was narrow, which was consistent with the characteristics of living? anionic polymerization. BMTFP has a strong ability to regulate the microstructure of styrene-butadiene copolymer. When the polymerization temperature was 50℃ and the BMTFP/ n-BuLi (mole ratio) was 2/1, the content of 1,2- structure in styrene-butadiene copolymer can reach 69.7%, and the content of styrene microblock tends to 0. Under the same experimental condition, BMTFP, ETE and DTHFP were at the same level in terms of their ability to regulate the microstructure of styrene butadiene copolymers. However, the raw materials required for BMTFP synthesis were bio-based platform compounds. From the aspects of environmental protection, energy security and potential economic value, BMTFP has a broad development prospect in the preparation of solution styrene butadiene rubber with high vinyl content.